Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Use the free IDT OligoAnalyzer™ software to help ensure that primer and probe sets lack hairpins and homo- and heterodimer interactions. From there, you can link directly to the NCBI BLAST™ tool to further analyze possible sequence interactions. For help with using the BLAST tool, see Using BLAST to find primers—Tips from IDT.
For more information on setting up multiplex qPCR assays, see the article Recommended dye combinations for multiplex qPCR.