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Libraries prepared using xGen PCR-Free Adapters for Ultima must be quantified prior to sequencing by qPCR using library quantification primers and standards available from Ultima (UG Library Quantification Kit, Ultima part number L09) and a 2X SYBR® qPCR master mix which must be purchased separately.
Quantification using fluorometric or electrophoretic methods are not suitable for quantifying libraries prepared using xGen PCR-Free Adapters for Ultima.