Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
There could be many different explanations for not getting any amplification. Assay design is fundamental to qPCR success.
Please see Primer design tools for PCR & qPCR for more information about designing primers and probes for qPCR analysis.