Frequently asked questions

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How do I incorporate a mutation into my donor template using the Alt-R™ HDR Design Tool?

A mutation, such as an insertion, deletion, single nucleotide polymorphism (SNP), or multiple nucleotide polymorphism (MNP), can be incorporated in 2 different ways in the HDR Design Tool:

  1. Enter the sequence to be inserted into the Mutation text box and this sequence will be incorporated at the location marked by the 2 sliders.
  2. Alternatively, move the sliders apart, and click on the bases between the sliders to change the sequence of each base, as needed.

Categories:
CRISPR genome editing

Tags:
crispr alt-r cas9 mutagenesis